Attenuation of the Trp (Tryptophan) Operon

Prokaryotic Regulation Table of Contents

Along with the 5 genes (trpE, trpD, trpC, trpB and trpA) coded by the trp operon there is a short protein-coding region at the 5' end of the operon called trpL. This does not code for a functional product but the very process of transcribing and then translating this sequence is the basis of another level of regulation of the operon (besides negative control by TrpR). This is regulation by attenuation. The basic idea is that even if transcription initiates when trp levels are high (which can happen since TrpR will occasionally come free from the Operator sequence) transcription will be terminated early (attenuated).

The process is fairly complicated and I will not give a diagram here but you can watch the video below. The basis of attenuation is as follows:

Therefore:

Low Trp = Ribosome Stalling = No termination structure = No Attenuation = EXPRESSION

High Trp = NO Ribosome Stalling = Termination structure = Attenuation = NO EXPRESSION


Hopefully this video will help:



Prokaryotic Regulation Table of Contents